Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay.
